Difference between revisions of "Part:BBa K3064022"
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This composite parts can be used to test the degradation of  some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.<Br/>
 
This composite parts can be used to test the degradation of  some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.<Br/>
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[[File:HA-tag--mmuTrim21.png|130px|]]
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[[File:Trim21.png|130px|]]
  
 
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Revision as of 00:07, 29 September 2019


HA-tag->mmuTrim21

This part can be used to test some certain expressed proteins that can bind with mmuTrim21.

Usage and Biology

HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used as a general epitope tag in expression vectors. It can facilitate the detection, isolation and purification of target proteins.

Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.

This composite parts can be used to test the degradation of some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.

HA-tag--mmuTrim21.png

Trim21.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 110
    Illegal BamHI site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]