Difference between revisions of "Part:BBa K2973009:Experience"

(→‎Applications of BBa_K2973009)
(→‎Applications of BBa_K2973009)
Line 6: Line 6:
 
===Applications of BBa_K2973009===
 
===Applications of BBa_K2973009===
 
In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes.
 
In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes.
 +
 
RPA reaction
 
RPA reaction
 
<html>
 
<html>
Line 15: Line 16:
 
       <img src="https://static.igem.org/mediawiki/parts/3/31/Thessaly2019_RPA_IS6110_Gel.jpeg" width="251"
 
       <img src="https://static.igem.org/mediawiki/parts/3/31/Thessaly2019_RPA_IS6110_Gel.jpeg" width="251"
 
         height="225">
 
         height="225">
 +
  </body>
 +
</html>
 +
 +
PCR reaction
 +
<html>
 +
  <head>
 +
      <title>HTML img Tag</title>
 +
  </head>
 +
 +
  <body>
 +
      <img src="https://static.igem.org/mediawiki/parts/e/e0/Thessaly_2019_PCR_IS6110_Gel.jpeg" width="570"
 +
        height="701">
 
   </body>
 
   </body>
 
</html>
 
</html>

Revision as of 18:47, 28 September 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2973009

In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes.

RPA reaction HTML img Tag

PCR reaction HTML img Tag

User Reviews

UNIQed85794419f572c4-partinfo-00000002-QINU UNIQed85794419f572c4-partinfo-00000003-QINU