Difference between revisions of "Part:BBa K2973013"
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Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. Upstream of the coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 5’ end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single stranded region (36bp) that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the coding gene. | Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. Upstream of the coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 5’ end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single stranded region (36bp) that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the coding gene. | ||
| + | This trigger sequence was derived from the 16S rRNA sequence of Geobacillus kaustophilus. | ||
| + | |||
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Revision as of 16:32, 28 September 2019
Trigger for Toehold switch 13 Geobacillus kaustophilus
Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. Upstream of the coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 5’ end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single stranded region (36bp) that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the coding gene. This trigger sequence was derived from the 16S rRNA sequence of Geobacillus kaustophilus.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]