Difference between revisions of "Part:BBa K2938007"

 
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Cyt1Aa Bti toxin with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
 
Cyt1Aa Bti toxin with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
  
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===Usage and Biology===
 
===Usage and Biology===
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The Cyt1Aa is a toxin with larvicidal effect on mosquitoes larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.
  
 
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Revision as of 12:31, 28 September 2019


Cyt1Aa With Strep Tag

Cyt1Aa Bti toxin with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Usage and Biology

The Cyt1Aa is a toxin with larvicidal effect on mosquitoes larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]