Difference between revisions of "Part:BBa K2938004"

 
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<partinfo>BBa_K2938004 short</partinfo>
 
<partinfo>BBa_K2938004 short</partinfo>
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===Description===
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p20 is a 20 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). It is a chaperone, which increases the production of Cry11Aa while expressed, and leads to higher mosquito larvicidal activity [2].
  
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P20
 
  
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===Amplification===
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===Usage and Biology===
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[[File:P20 PCR.jpeg|200px|thumb|left|PCR  amplification of P20 by PCR.]]
  
 
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Revision as of 10:30, 28 September 2019


P20

Description

p20 is a 20 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). It is a chaperone, which increases the production of Cry11Aa while expressed, and leads to higher mosquito larvicidal activity [2].


Amplification

PCR amplification of P20 by PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]