Difference between revisions of "Part:BBa K2918023"
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===Characterization===
 
===Characterization===
 +
 +
For characterizing our constructs we used PURE(Protein synthesis Using Recombinant Elements) system. This is an E.coli based cell-free protein synthesis system and it contains all the elements to make in vitro translation-transcription possible.
  
 
Protein gel + Mass Spectrometry + transformation + sequencing(gotag check pcr)
 
Protein gel + Mass Spectrometry + transformation + sequencing(gotag check pcr)

Revision as of 06:56, 28 September 2019


Weak T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator

This part consists of a T7 promotor, a universal Ribosome Binding Site (RBS), a Coding DNA Sequence (CDS) coding for the DSB p6 and a Wild Type T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 68
    Illegal BsaI.rc site found at 94

Overview

This protein is one of the 4 components needed for orthogonal replication

Characterization

For characterizing our constructs we used PURE(Protein synthesis Using Recombinant Elements) system. This is an E.coli based cell-free protein synthesis system and it contains all the elements to make in vitro translation-transcription possible.

Protein gel + Mass Spectrometry + transformation + sequencing(gotag check pcr)

Toxicity