Difference between revisions of "Part:BBa K3198008"

Revision as of 10:34, 27 September 2019

pBAD-HicB

This part contains the arabinose inducible promoter pBAD and antitoxin component HicB of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death.

Description

This part contains the antitoxin component of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death.

Usage

Team NUS Singapore 2019 has added a new biobrick (BBa_K3198008) into the iGEM repository this year. This biobrick was found to possess the ability to neutralize the effect of BBa_K3198000 and therefore functions as an antitoxin. For this reason, team NUS Singapore 2019 used this biobrick as part of their sleep-wake module to control the growth of E. coli. More specifically, to overcome the pre-induced dormant state of these cells.

Biology

HicB is from hicAB locus of Escherichia coli K-12. HicB functions as an mRNA interferase antitoxin. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. HicB neutralizes HicA and therefore functions as an antitoxin. HicB could resuscitate cells inhibited by HicA.

References

Jorgensen, M. G., Pandey, D. P., Jaskolska, M., & Gerdes, K. (2008). HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea. Journal of Bacteriology, 191(4), 1191–1199. doi: 10.1128/jb.01013-08

Maisonneuve, E., Shakespeare, L. J., Jørgensen, M. G., & Gerdes, K. (2011). Bacterial persistence by RNA endonucleases. Proceedings of the National Academy of Sciences, 108(32), 13206–13211. doi: 10.1073/pnas.1100186108

Source

BBa_K3198001 originated from E. coli K12 and its sequence was synthesized by IDT.

Design Considerations

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 204
Illegal BsaI.rc site found at 244

@@ Line 9: / Line 9: @@
 ===Usage===
-Team NUS Singapore 2019 has added a new biobrick (BBa_K3198001) into the iGEM repository this year. This biobrick was found to possess the ability to neutralize the effect of BBa_K3198000 and therefore functions as an antitoxin. For this reason, team NUS Singapore 2019 used this biobrick as part of their sleep-wake module to control the growth of E. coli. More specifically, to overcome the pre-induced dormant state of these cells.
+Team NUS Singapore 2019 has added a new biobrick (BBa_K3198008) into the iGEM repository this year. This biobrick was found to possess the ability to neutralize the effect of BBa_K3198000 and therefore functions as an antitoxin. For this reason, team NUS Singapore 2019 used this biobrick as part of their sleep-wake module to control the growth of E. coli. More specifically, to overcome the pre-induced dormant state of these cells.
 ===Biology===
@@ Line 18: / Line 18: @@
 ===References===
 Jorgensen, M. G., Pandey, D. P., Jaskolska, M., & Gerdes, K. (2008). HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea. Journal of Bacteriology, 191(4), 1191–1199. doi: 10.1128/jb.01013-08
-Maisonneuve, E., Shakespeare, L. J., Jørgensen, M. G., & Gerdes, K. (2011). Bacterial persistence by RNA endonucleases. Proceedings of the National Academy of Sciences, 108(32), 13206–13211. doi: 10.1073/pnas.1100186108
+<br><br>Maisonneuve, E., Shakespeare, L. J., Jørgensen, M. G., & Gerdes, K. (2011). Bacterial persistence by RNA endonucleases. Proceedings of the National Academy of Sciences, 108(32), 13206–13211. doi: 10.1073/pnas.1100186108