Difference between revisions of "Part:BBa K2916023"

 
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<partinfo>BBa_K2916023 short</partinfo>
 
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This part is used for expression of IleRS needed for the PURE and OnePot PURE cell-free systems.
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===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
  
 
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Revision as of 22:42, 26 September 2019


Expression of IleRS in E.coli

This part is used for expression of IleRS needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2996
    Illegal NotI site found at 2874
    Illegal NotI site found at 2936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 937
    Illegal BamHI site found at 1440
    Illegal XhoI site found at 2945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 2015
    Illegal AgeI site found at 2474
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1559