Difference between revisions of "Part:BBa K2916035"

 
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This part is used for expression of IF2 needed for the PURE and OnePot PURE cell-free systems.
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===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2916034 parameters</partinfo>
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<partinfo>BBa_K2916035 parameters</partinfo>
 
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Revision as of 18:08, 25 September 2019


Expression of IF2 in E.coli


This part is used for expression of IF2 needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1760
    Illegal AgeI site found at 371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1091
    Illegal SapI site found at 1026