Difference between revisions of "Part:BBa K2916014:Design"
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The protein was expressed in pQE30 in M15 E.coli strain. | The protein was expressed in pQE30 in M15 E.coli strain. | ||
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===Source=== | ===Source=== | ||
Revision as of 17:12, 25 September 2019
Expression of AlaRS in E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 573
Illegal BglII site found at 780
Illegal BamHI site found at 746 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1184
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2584
Illegal BsaI site found at 2761
Illegal BsaI.rc site found at 1133
Illegal SapI site found at 1868
Design Notes
The protein was expressed in pQE30 in M15 E.coli strain.
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
http://www.addgene.org/124103/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.