Difference between revisions of "Part:BBa K2927066"
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The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3. | The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3. | ||
Revision as of 14:41, 25 September 2019
T7 promoter-guide RNA-crRNA 6-mutated crRNA 3
The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]