Difference between revisions of "Part:BBa K2992032"

 
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RBS region for the <i>ctfA</i> gene from <i>C. botulinum</i>.
 
RBS region for the <i>ctfA</i> gene from <i>C. botulinum</i>.
  
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===Usage and Biology===
 
===Usage and Biology===
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This parts entry represents the RBS for the <i>ctfA</i> gene in <i>C. botulinum</i>. The CtfAB complex is central to the production of biotechnologically relevant solvents in <i>C. acetobutylicum</i> such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both <i>C. acetobutylicum</i> and <i>C. botulinum</i>-derived <i>ctfAB</i>.<br><br>
 
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===Characterisation===
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Incoming.
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992032 SequenceAndFeatures</partinfo>
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===References===
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Cornillo et al., 1997
  
  

Revision as of 14:07, 25 September 2019


RBS region for the ctfA gene from C. botulinum

RBS region for the ctfA gene from C. botulinum.

Usage and Biology

This parts entry represents the RBS for the ctfA gene in C. botulinum. The CtfAB complex is central to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

Incoming. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cornillo et al., 1997