Difference between revisions of "Part:BBa K2927014"

Revision as of 13:21, 25 September 2019

DNA template of crRNA 5 for LbCas12a

crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).

When crRNA 5 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 5 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 5 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 5 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2927014 short</partinfo>
-CrRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and activates it. When crRNA binds on Cas12a protein, it makes Cas12a protein cleavage not only target sequence but also non-specific ssDNA. In our project, we use crRNA for detecting the African swine fever virus (ASFV). When crRNA 5 binds on Cas12a protein, the protein can not only detect the specific sequence of ASFV but also cleavage DNA of ASFV and non-specific ssDNA. By the way, though crRNA 5 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 3 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 5 is too short to become a functional protein. There is no concentration for spread out ASFV of our project.
+crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).
+When crRNA 5 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 5 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 5 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 5 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project.
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