Difference between revisions of "Part:BBa K3187020"

Line 5: Line 5:
 
The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa.
 
The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa.
  
 +
<html>
 +
<p style="text-align:center;"><img src="https://static.igem.org/mediawiki/parts/7/7c/T--TU_Darmstadt--FRETassay.png" alt="Test FRET“ width="400" height="350"></p>
 +
<p align="center"><b>Figure 2:</b> TEST TEST TEST </p> <br>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:02, 25 September 2019


Tobacco Vein Mottling Virus Protease Cleavage Site

The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa.

Test FRET“ width=

Figure 2: TEST TEST TEST


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]