Difference between revisions of "Part:BBa K3195003:Design"
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===References=== | ===References=== | ||
+ | He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B | ||
+ | http://doi.org/10.1098/rspb.2018.0438 |
Revision as of 15:25, 24 September 2019
His-tagged Cathepsin B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 369
Illegal XhoI site found at 958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because Cathespin B gene is in eukaryotes,we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add 6X His at its tail.In addition,we cut off the signal peptide.
Source
Cathespin B gene from Branchiostoma belcheri tsingtauense
References
He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B http://doi.org/10.1098/rspb.2018.0438