Difference between revisions of "Part:BBa K2933277"

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<partinfo>BBa_K2933277 short</partinfo>
 
<partinfo>BBa_K2933277 short</partinfo>
  
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This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+BcII-194) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
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This part consists of RBS, Linker h, protein coding sequence(His+Linker f+BcII-194) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
  
  
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===Usage and Biology===
 
===Usage and Biology===
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This composite part is made up with three basic parts(RBS, Linker h and T7 terminator)and a composite part(His+Linker f+BcII-194). It encodes a protein which is BcII fused with His tag. The fusion protein is about 28.1 kD. It is convenient for us to purify our target protein.
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This composite part is made up with six basic parts(RBS , Linker h, His, Linker f ,  BcII-194 and T7 terminator ). It encodes a protein which is BcII fused with His tag. The fusion protein is about 28.1 kD. It is convenient for us to purify our target protein.
  
 
===Molecular cloning===
 
===Molecular cloning===

Latest revision as of 14:21, 24 September 2019


RBS+Linker h+His+Linker f+BcII-194+T7 terminator

This part consists of RBS, Linker h, protein coding sequence(His+Linker f+BcII-194) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 907
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with six basic parts(RBS , Linker h, His, Linker f , BcII-194 and T7 terminator ). It encodes a protein which is BcII fused with His tag. The fusion protein is about 28.1 kD. It is convenient for us to purify our target protein.

Molecular cloning

First,we obtained BcII by PCR.

BcII-194 PCR.jpeg
Figure 1. The PCR result of BcII.

References

Sung-Kun Kim, Mara Demuth, Sara R. Schlesinger, Sung Joon Kim, Jonathan Urbanczyk, Robert W. Shaw & Hyunshun Shin (2016) Inhibition of Bacillusanthracis metallo-β- lactamase by compounds with hydroxamic acid functionality, Journal of Enzyme Inhibition and Medicinal Chemistry, 31:sup4, 132-137, DOI: 10.1080/14756366.2016.1222580