Difference between revisions of "Part:BBa K2933283"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This composite part is made up with | + | This composite part is made up with three basic parts(RBS, Linker h and T7 terminator)and a composite part(His+Linker f+IMP-71). It encodes a protein which is IMP-71 fused with His tag. The fusion protein is about 27.2 kD. It is convenient for us to purify our target protein. |
===Molecular cloning=== | ===Molecular cloning=== | ||
Revision as of 12:57, 24 September 2019
RBS+Linker h+His+Linker f+IMP-71+T7 terminator
This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+IMP-71) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 852
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 877
Illegal PstI site found at 852 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 852
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 852
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This composite part is made up with three basic parts(RBS, Linker h and T7 terminator)and a composite part(His+Linker f+IMP-71). It encodes a protein which is IMP-71 fused with His tag. The fusion protein is about 27.2 kD. It is convenient for us to purify our target protein.
Molecular cloning
First, we used the vector pET28a to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. The PCR result of IMP-71.