Difference between revisions of "Part:BBa K2918023"
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<partinfo>BBa_K2918023 short</partinfo> | <partinfo>BBa_K2918023 short</partinfo> | ||
| − | + | This part consists of a T7 promotor, a universal Ribosome Binding Site(RBS), a Coding DNA Sequence (CDS) coding for the DSB p6 and a Wild type terminator T7. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This protein is one of the 4 components needed for orthogonal replication | ||
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| + | ===Characterization=== | ||
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Revision as of 12:28, 24 September 2019
Weak T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator
This part consists of a T7 promotor, a universal Ribosome Binding Site(RBS), a Coding DNA Sequence (CDS) coding for the DSB p6 and a Wild type terminator T7.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 292
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 68
Illegal BsaI.rc site found at 94