Difference between revisions of "Part:BBa K3257100"
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This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time. | This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time. | ||
| − | According to our experiment, our | + | According to our experiment, our Lac operon is improved in the following three main aspects. |
'''Lower Response to Arabinose''' | '''Lower Response to Arabinose''' | ||
Revision as of 17:43, 23 September 2019
Improved LacI Expression System
This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time.
According to our experiment, our Lac operon is improved in the following three main aspects.
Lower Response to Arabinose
Same Level of Expression when Induced by IPTG
Lower Uninduced Leakage
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1258
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1258
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1258
- 1000COMPATIBLE WITH RFC[1000]
Reference
AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.