Difference between revisions of "Part:BBa K2951008:Design"
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===References=== | ===References=== | ||
+ | Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64 | ||
+ | (https://doi.org/10.1016/j.jviromet.2013.10.035) |
Latest revision as of 17:30, 23 September 2019
LysRS-Hemagglutinin fusion protein
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 275
Illegal NotI site found at 1256 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 864
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1845
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For Hemagglutinin 1.Codon optimization: We optimized the sequence's codon usage for E.coli.
2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.
3.Deletion of transmembrane domain: To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.
Source
Influenza A virus (A/WSN/1933 TS61(H1N1) obtained from NCBI GenBank(CY010788.1).
References
Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64 (https://doi.org/10.1016/j.jviromet.2013.10.035)