Difference between revisions of "Part:BBa K3257100:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | lacIq promoter and rrnB terminator are both the endogenous biobricks that can be found in Escherichia coli or other bacteria. | + | lacIq promoter and rrnB terminator are both the endogenous biobricks that can be found in Escherichia coli or other bacteria so that this system works well in E.coli BL21(DE3). |
===Source=== | ===Source=== | ||
Latest revision as of 17:30, 23 September 2019
Improved LacI Expression System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1258
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1258
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1258
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
lacIq promoter and rrnB terminator are both the endogenous biobricks that can be found in Escherichia coli or other bacteria so that this system works well in E.coli BL21(DE3).
Source
Combined from BBa_K3257003, BBa_K3257045, BBa_K3257200 and BBa_K3257020.
References
AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.