Difference between revisions of "Part:BBa K3257100"
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<partinfo>BBa_K3257100 short</partinfo> | <partinfo>BBa_K3257100 short</partinfo> | ||
| − | This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). | + | This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time. |
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Revision as of 17:24, 23 September 2019
Improved LacI Expression System
This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1258
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1258
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1258
- 1000COMPATIBLE WITH RFC[1000]