Difference between revisions of "Part:BBa K3257100"

 
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This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3).  
  
 
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Revision as of 17:21, 23 September 2019


Improved LacI Expression System

This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1258
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]