Difference between revisions of "Part:BBa K2927004"
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<partinfo>BBa_K2927004 short</partinfo>
 
<partinfo>BBa_K2927004 short</partinfo>
  
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TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli.
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TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. This process makes the Cas12a protein can be collected in the solution.  
  
 
'''Reference'''<br>
 
'''Reference'''<br>

Revision as of 15:51, 23 September 2019


Tobaco Etch Virus (TEV) motif

TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. This process makes the Cas12a protein can be collected in the solution.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]