Difference between revisions of "Part:BBa K2916006"

 
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<partinfo>BBa_K2916006 short</partinfo>
 
<partinfo>BBa_K2916006 short</partinfo>
  
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Glutaminyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.  
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Lysyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.  
  
  
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
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Revision as of 12:59, 23 September 2019


LysRS protein equipped with a 6x HIS affinity tag

Lysyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1121
    Illegal XhoI site found at 1516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475