Difference between revisions of "Part:BBa K2933211"
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==Usage and Biology=== | ==Usage and Biology=== | ||
| − | This composite part is made up with | + | This composite part is made up with seven basic parts, the His tag, T7 promoter, RBS and our target protein MUS-2. It encodes a protein which is MUS-2 fused with His tag. The fusion protein is about 28.3 kD. In order to gain the highly purified target protein, we add His tag in N-terminal of MUS-2 . It is convenient for us to purify our target protein.<br> |
===Molecular cloning=== | ===Molecular cloning=== | ||
We used the vector pGEX-6p-1 to construct our expression plasmid. | We used the vector pGEX-6p-1 to construct our expression plasmid. | ||
Revision as of 12:56, 23 September 2019
T7 promoter+RBS b+Linker h+His+Linker f+MUS-2+T7 terminator
This part consists of T7 promoter, RBS and protein coding sequence（His+Linker f+MUS-2），and the biological module can be built into E.coli for protein expression.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 169
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology=
This composite part is made up with seven basic parts, the His tag, T7 promoter, RBS and our target protein MUS-2. It encodes a protein which is MUS-2 fused with His tag. The fusion protein is about 28.3 kD. In order to gain the highly purified target protein, we add His tag in N-terminal of MUS-2 . It is convenient for us to purify our target protein.
Molecular cloning
We used the vector pGEX-6p-1 to construct our expression plasmid.
Figure 1. The PCR result of MUS-2.
After verification, it was determined that the construction is successful.