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| − | __NOTOC__
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| − | <partinfo>BBa_K581005 short</partinfo>
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| − | '''SgrS'''(sugar transport-related sRNA)'''(wt)''' is a small RNA regulator that help cells recover from glucose-phosphate stress by base pairing with '''ptsG(wt)'''[https://parts.igem.org/Part:BBa_K581006] mRNA. SgrS regulates ptsG mRNA by short, imperfect base-pairing interactions; as a result, the expression of PtsG is repressed.
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| − | Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. These results indicate that it is the base pairing of the two RNAs rather than particular nucleotides that is important for SgrS action. They have also illustrated that sequence outside this region, even though complementary, is rather dispensable for the efficient silencing (Kawamoto et al., 2006). This makes mutant ptsG/SgrS pairs orthogonal to genetic context of the host cell. Therefore we choose this couple of conjugate mRNA/sRNA as the foundation of our comparator device design.
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| − | '''SgrS(wt)''' in this part will be constitutively expressed in E.coli after transformation processing.
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| − | [[Image:Mechanism.png]]
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| − | ''' Fig.1''' Sequence alignment of wildtype ptsG/SgrS pair and its mutant complementary pairs.
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K581005 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K581005 parameters</partinfo>
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| − | //only for last years page
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| | ===Link to our Improved Part=== | | ===Link to our Improved Part=== |
| − | <a href="https://parts.igem.org/Part:BBa_K3198005" target="_blank" >BBa_K3198005</a>
| + | https://parts.igem.org/Part:BBa_K3198005 |
Revision as of 05:12, 23 September 2019
Following is a contribution to this part, by the NUS_Singapore 2019 team.
Group
NUS_Singapore 2019
Author
Sheena Chan
Summary and Uploads
Link to our Improved Part
https://parts.igem.org/Part:BBa_K3198005
