Difference between revisions of "Part:BBa K2957007"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2957007 short</partinfo>
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<partinfo>BBa_K2957099 short</partinfo>
  
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"Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
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Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
  
 
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression  
 
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression  
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What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.  
 
What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.  
  
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2957007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2957007 parameters</partinfo>
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<partinfo>BBa_K2957099 parameters</partinfo>
 
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Revision as of 01:36, 23 September 2019


hEF1a, IL-8 (CXCL-8)

Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2684
    Illegal BamHI site found at 3290
    Illegal BamHI site found at 3569
    Illegal XhoI site found at 3083
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
    Illegal NgoMIV site found at 197
    Illegal NgoMIV site found at 2818
    Illegal AgeI site found at 2025
    Illegal AgeI site found at 2196
    Illegal AgeI site found at 3673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2073
    Illegal SapI.rc site found at 9