Difference between revisions of "Part:BBa K2957007"

Revision as of 01:35, 23 September 2019

hEF1a, IL-8 (CXCL-8), NeonGreen Tag

"Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1256
Illegal PstI site found at 699
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 699
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 473
Illegal BamHI site found at 715
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1256
Illegal PstI site found at 699
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1256
Illegal PstI site found at 699
Illegal AgeI site found at 81
Illegal AgeI site found at 1262
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 "Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
 What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression
 What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
 How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."