Difference between revisions of "Part:BBa K2926004"

 
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__NOTOC__
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<partinfo>BBa_K2926004 short</partinfo>
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==Usage and Biology==
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<p>
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This is the constitutive protomtor for SNR52 form <i>Saccharomyces cerevisiae</i>.
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SNR52 is a small nucleolar RNA (snoRNA), belonging to the C/D box of snoRNAs,
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which are associated with mehtylation.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2926004 SequenceAndFeatures</partinfo>
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__TOC__
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2926003 parameters</partinfo>
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<!-- -->
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==Plasmid Design==
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  For the analysis and characterization of the SNR52 Promotor <a href="XXX">XXXX</a>
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  combiantion with the SUP4 Terminaror <a href="XXX">XXXX</a>,
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  sfGFP <a href="XXX">XXXX</a> was cloned beteen them using Gibson Assembly.
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  The SNR52 ans TPs1 where optiaied as a gBlock from IDT.
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</html>
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===Sequencing Results===
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The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
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</html>
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==Characterisation==
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We created a new <a href="https://2019.igem.org/Team:Bielefeld-CeBiTec/mCherry">protocol for the calibration of mCherry</a> measurements with TexasRed,
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based on the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>,
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We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures.
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<br>
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See <a href="XXX">BBa_K2926072</a> and <a href="XXX">BBa_K2926073</a> for the characterization results.
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</html>
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==References==
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Revision as of 21:59, 22 September 2019


SNR52 yeast promotor


Usage and Biology

This is the constitutive protomtor for SNR52 form Saccharomyces cerevisiae. SNR52 is a small nucleolar RNA (snoRNA), belonging to the C/D box of snoRNAs, which are associated with mehtylation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Design

For the analysis and characterization of the SNR52 Promotor XXXX combiantion with the SUP4 Terminaror XXXX, sfGFP XXXX was cloned beteen them using Gibson Assembly. The SNR52 ans TPs1 where optiaied as a gBlock from IDT.

Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.


Characterisation

We created a new protocol for the calibration of mCherry measurements with TexasRed, based on the standard iGEM protocoll for GFP fluorescence calibration, We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See BBa_K2926072 and BBa_K2926073 for the characterization results.


References