Difference between revisions of "Part:BBa K3125000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Silent mutations made to remove illegal cut sites. kpn1 and hindiii sites added for cloning.
+
GenBank AY731700.1 with 6 silent mutations made to decrease GC content during DNA
−
 
+
synthesis. 4 illegal PstI sites and 1 illegal EcoRI site removed
−
 
+
  
 
===Source===
 
===Source===

Revision as of 21:29, 22 September 2019


trehalose-6-phosphate synthase from Rhizobium etli CFN 42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 1102
    Illegal NgoMIV site found at 1357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 727


Design Notes

GenBank AY731700.1 with 6 silent mutations made to decrease GC content during DNA synthesis. 4 illegal PstI sites and 1 illegal EcoRI site removed

Source

R. etli cfn42

References