Difference between revisions of "Part:BBa K137054"
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This is the folate synthesis gene ''folKE'', one of five genes (''folB'', ''folKE'', ''folP'', ''folC'', ''folA'') involved in the folate synthesis operon. In this construct, ''folKE'' has been inserted behind the strong promoter J23100, the strong RBS B0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.  
 
This is the folate synthesis gene ''folKE'', one of five genes (''folB'', ''folKE'', ''folP'', ''folC'', ''folA'') involved in the folate synthesis operon. In this construct, ''folKE'' has been inserted behind the strong promoter J23100, the strong RBS B0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.  
  
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The purpose of this construct is to test the effects of FolKE (a fusion gene for GTP cyclohydrolase I (FolE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (FolK)) overexpression on total folate production in ''Escherichia coli'', with the goal of increasing total folate production.  
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The purpose of this construct is to test the effects of FolKE (a bifunctional GTP cyclohydrolase I (FolE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (FolK)) overexpression on total folate production in ''Escherichia coli'', with the goal of increasing total folate production.  
  
 
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Revision as of 23:10, 23 August 2008

Constitutive folKE expression construct

This is the folate synthesis gene folKE, one of five genes (folB, folKE, folP, folC, folA) involved in the folate synthesis operon. In this construct, folKE has been inserted behind the strong promoter J23100, the strong RBS B0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.

The purpose of this construct is to test the effects of FolKE (a bifunctional GTP cyclohydrolase I (FolE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (FolK)) overexpression on total folate production in Escherichia coli, with the goal of increasing total folate production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]