Difference between revisions of "Part:BBa K137054"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K137054 short</partinfo> | <partinfo>BBa_K137054 short</partinfo> | ||
− | This is the folate synthesis gene folKE, one of five genes (folB, folKE, folP, folC, folA) involved in the folate synthesis operon. In this construct, folKE has been inserted behind the strong promoter | + | This is the folate synthesis gene ''folKE'', one of five genes (''folB'', ''folKE'', ''folP'', ''folC'', ''folA'') involved in the folate synthesis operon. In this construct, ''folKE'' has been inserted behind the strong promoter J23100, the strong RBS B0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG. |
− | The purpose of this construct is to test the effects of | + | The purpose of this construct is to test the effects of FolKE (a fusion gene for GTP cyclohydrolase I (FolE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (FolK)) overexpression on total folate production in ''Escherichia coli'', with the goal of increasing total folate production. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 23:10, 23 August 2008
Constitutive folKE expression construct
This is the folate synthesis gene folKE, one of five genes (folB, folKE, folP, folC, folA) involved in the folate synthesis operon. In this construct, folKE has been inserted behind the strong promoter J23100, the strong RBS B0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.
The purpose of this construct is to test the effects of FolKE (a fusion gene for GTP cyclohydrolase I (FolE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (FolK)) overexpression on total folate production in Escherichia coli, with the goal of increasing total folate production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]