Difference between revisions of "Part:BBa K137055"

Revision as of 23:09, 23 August 2008

Constitutive pabA expression construct

pabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K137055 short</partinfo>
-PabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter j23100 and the strong RBS b0034, and in front of the terminator b0015. Currently it is in the inducible copy plasmid psb2k3, which can be induced to high copy via IPTG.
+''pabA'' is one of three genes (''pabA'', ''pabB'', ''pabC'') involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.