Difference between revisions of "Part:BBa K2933143"
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This composite part is made up with three basic parts, the HiS tag, the thrombin restriction site and our target protein Elbla2-1. It encodes a protein which is Elbla2-1 fused with His tag. The fusion protein is about 27.5 kD. It is convenient for us to purify our target protein.
 
This composite part is made up with three basic parts, the HiS tag, the thrombin restriction site and our target protein Elbla2-1. It encodes a protein which is Elbla2-1 fused with His tag. The fusion protein is about 27.5 kD. It is convenient for us to purify our target protein.
 
===Molecular cloning===
 
===Molecular cloning===
−
First, we used the vector pET28B-Sumo to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br>
+
First, we used the vector pET28a to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br>
 
<p style="text-align: center;">
 
<p style="text-align: center;">
 
   [[File:Elbla pcr.png]]<br>
 
   [[File:Elbla pcr.png]]<br>
 
'''Figure 1.'''  The PCR result of Elbla2-1.
 
'''Figure 1.'''  The PCR result of Elbla2-1.

Revision as of 04:15, 22 September 2019


His+Linker f+beta-lactamase II [Erythrobacter litoralis HTCC2594]

This part encodes the fusion protein of His tag and beta-lactamase II to promote the expression and purification of beta-lactamase II [Erythrobacter litoralis HTCC2594].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with three basic parts, the HiS tag, the thrombin restriction site and our target protein Elbla2-1. It encodes a protein which is Elbla2-1 fused with His tag. The fusion protein is about 27.5 kD. It is convenient for us to purify our target protein.

Molecular cloning

First, we used the vector pET28a to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.

Elbla pcr.png
Figure 1. The PCR result of Elbla2-1.