Difference between revisions of "Part:BBa K2933262"

 
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<partinfo>BBa_K2933262 parameters</partinfo>
 
<partinfo>BBa_K2933262 parameters</partinfo>
 
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===Usage and Biology===
 +
Bacteria with IMP-type enzymes have spread through out the world, and the IMP group now has more than 50 variants
 +
These enzymes possess a broad substrate specificity and a high affinity for cephalosporins and carbapenems but a low activity toward temocillin<br>
 +
==References==
 +
 +
 +
===Molecular cloning===
 +
First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br>
 +
<p style="text-align: center;">
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  [[File:IMP-71-PCR.png|400px]]<br>
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'''Figure 1.'''  Left: The PCR result of IMP-71. Right: The verification results by enzyme digestion.<br>
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</p>
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After verification, it was determined that the construction is successful. We converted the plasmid to ''E. coli'' BL21(DE3) for expression and purification.<br>

Revision as of 11:31, 21 September 2019


RBS b+Linker h+His+Linker a+Sumo+Linker b+IMP-71+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker a+Sumo+Linker b+IMP-71) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 1121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal NheI site found at 75
    Illegal NheI site found at 1146
    Illegal PstI site found at 1121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 187
    Illegal BamHI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 1121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Bacteria with IMP-type enzymes have spread through out the world, and the IMP group now has more than 50 variants These enzymes possess a broad substrate specificity and a high affinity for cephalosporins and carbapenems but a low activity toward temocillin

References

Molecular cloning

First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.

IMP-71-PCR.png
Figure 1. Left: The PCR result of IMP-71. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful. We converted the plasmid to E. coli BL21(DE3) for expression and purification.