Difference between revisions of "Part:BBa K3182300"
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− | [[File:T--Linkoping_Sweden--mngimprovementsequence.png|900px|thumb|left|<b>Figure 1.</b> A schematic representation of the improvement. Where the promotor ( | + | [[File:T--Linkoping_Sweden--mngimprovementsequence.png|900px|thumb|left|<b>Figure 1.</b> A schematic representation of the improvement. Where the promotor (purple, pBAD) of the previous biobrick (BBa_K2671000) has been exchanged to pT7. Translation enhancing '5-UTR containing g10-L RBS (BBa_K1758100) has been added as well to the improvement. Together with removal of extra bases (named UTR in figure 1) which is non-coding. A BamHI site was added after the His-tag. The blue colors illustrate the protein coding part of the biobricks.]] |
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Revision as of 12:09, 20 September 2019
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 91
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
pT7-mNG
This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color. This part includes a T7 promotor as well as a 5'-UTR region for higher expression.
![](/wiki/images/8/82/T--Linkoping_Sweden--mngimprovementsequence.png)
Figure 1. A schematic representation of the improvement. Where the promotor (purple, pBAD) of the previous biobrick (BBa_K2671000) has been exchanged to pT7. Translation enhancing '5-UTR containing g10-L RBS (BBa_K1758100) has been added as well to the improvement. Together with removal of extra bases (named UTR in figure 1) which is non-coding. A BamHI site was added after the His-tag. The blue colors illustrate the protein coding part of the biobricks.
Usage and Biology