Difference between revisions of "Part:BBa K2916002"

 
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<partinfo>BBa_K2916002 short</partinfo>
 
<partinfo>BBa_K2916002 short</partinfo>
  
Asparaginyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.The His tag is used
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Glutamyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.  
  
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===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
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Revision as of 11:26, 20 September 2019


GluRS protein equipped with a 6x HIS affinity tag

Glutamyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1297
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 328