Difference between revisions of "Part:BBa K2916001:Design"
Konstantinos (Talk | contribs) (→Design Notes) |
Konstantinos (Talk | contribs) (→Design Notes) |
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The sequence was extracted from the pET21-GlnRS-His plasmid and expressed in the BL21(DE3) E.coli strain | The sequence was extracted from the pET21-GlnRS-His plasmid and expressed in the BL21(DE3) E.coli strain | ||
| − | Second stop codon was added | + | Second stop codon was added in compliance with iGEM standards. |
===Source=== | ===Source=== | ||
Revision as of 11:17, 20 September 2019
GlnRS protein equipped with a 6x HIS affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 54
Illegal BglII site found at 1002
Illegal XhoI site found at 1663 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1608
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was extracted from the pET21-GlnRS-His plasmid and expressed in the BL21(DE3) E.coli strain
Second stop codon was added in compliance with iGEM standards.
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
https://www.addgene.org/124108/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568 \
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.