Difference between revisions of "Part:BBa K3128023:Design"
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===Source=== | ===Source=== | ||
− | + | pKT25-ZIP plasmid from Euromedex BACTH kit was used (containing the T25 subpart and the leucine zipper gene). OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates. | |
===References=== | ===References=== |
Revision as of 21:36, 18 September 2019
OmpX-WT with Leucine Zipper and T25 subpart of Bordetella Pertussis AC under lactose promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1764
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 344
Design Notes
The signal peptide has a conformation enabling the protein addressing to the membrane. This sequence is cut after the translocation. If the OmpX gene is used without changes, the leucine zipper gene is present before the signal peptide, thus generating a cut in the fusion protein leucine-zipper-OmpX.
Source
pKT25-ZIP plasmid from Euromedex BACTH kit was used (containing the T25 subpart and the leucine zipper gene). OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.