Difference between revisions of "Part:BBa K2992024:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Unmodified from <i>C. perfringens</i> genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system.
+
Unmodified from <i>C. perfringens</i> genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the <i>bgaRL</i> system, all <i>bgaRL</i> entries are listed 5'-3'-exactly as found on the chromosome of <i>C. perfringens</i> to allow the correct topology when assembled as a composite part. 
  
  

Latest revision as of 13:16, 18 September 2019


5-UTR containing RBS for bgaL from C. perfringens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unmodified from C. perfringens genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the bgaRL system, all bgaRL entries are listed 5'-3'-exactly as found on the chromosome of C. perfringens to allow the correct topology when assembled as a composite part.


Source

C. perfringens genomic DNA

References