Difference between revisions of "Part:BBa K2992020:Design"

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===Design Considerations===
 
===Design Considerations===
  
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Unmodified from <i>C. perfringens</i> genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system.This regulator forms part of lactose inducible unit and is therefore listed in the 3’-5’-orientation to allow correct topology when applied to composite parts.
+
Unmodified from <i>C. perfringens</i> genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the <i>bgaRL</i> system, all <i>bgaRL</i> entries are listed 5'-3'-exactly as found on the chromosome of <i>C. perfringens</i> to allow the correct topology when assembled as a composite part.

Latest revision as of 13:14, 18 September 2019

Design Considerations

Unmodified from C. perfringens genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the bgaRL system, all bgaRL entries are listed 5'-3'-exactly as found on the chromosome of C. perfringens to allow the correct topology when assembled as a composite part.