Difference between revisions of "Part:BBa K2992027:Design"
 
Line 1: Line 1:
 
===Design Considerations===  
 
===Design Considerations===  
−
Amplified unmodified from the genome sequences of the host organisms. The <i>bgaRL</i> regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the <i>bgaRL</i> system many constituents are listed in the 3’-5’-orientation to allow correct topology when applied to composite parts.
+
Amplified unmodified from the genome sequences of the host organisms. The <i>bgaRL</i> regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the <i>bgaRL</i> system, all <i>bgaRL</i> entries are listed 5'-3'-exactly as found on the chromosome of <i>C. perfringens</i>

Latest revision as of 13:13, 18 September 2019

Design Considerations

Amplified unmodified from the genome sequences of the host organisms. The bgaRL regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the bgaRL system, all bgaRL entries are listed 5'-3'-exactly as found on the chromosome of C. perfringens