Difference between revisions of "Part:BBa K2992025"
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<partinfo>BBa_K2992025 short</partinfo>
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<partinfo>BBa_K29920125 short</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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This parts entry represents an integration module for the expression of <i>botR</i> from the <i>pyrE</i> locus of the <i>C. sporogenes</i> genome. This module comprises a strong clostridial terminator sequence T<i>fad</i> to prevent polar transcription from <i>pyrD</i> [https://parts.igem.org/Part:BBa_K2992013] and the <i>botR</i> gene of<i>C. botulinum</i> [https://parts.igem.org/Part:BBa_K2992002], under the regulatory control of its native promoter P<i>botR</i> [https://parts.igem.org/wiki/Part:BBa_K2992012] and its associated 5’-UTR which contains the RBS [https://parts.igem.org/Part:BBa_K2992014]. An additional strong clostridial terminator was included to prevent polar effects for the transcription of<i> pyrE</i> and any downstream genes on the chromosome of <i>C. sporogenes</i>. In our project we use the transcriptional regulator BotR to control the regulation of our volatile reporter operons (add comp part hyperlinks) and our fluorescent reporter FAST [] through interaction with its own promoter sequence P<i>botrR</i> [https://parts.igem.org/Part:BBa_K2992012] and P<i>ntnH</i> [https://parts.igem.org/Part:BBa_K2992001] whose genes are cognate members of the BotR regulon. Doing so allows us to use our surrogate host strain <i>C. sporogenes</i> as a model system for predicting botulinum neurotoxin production following food manufacture, through the detection of our chosen reporters.  
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This parts entry represents an integration module for the expression of <i>botR</i> from the <i>pyrE</i> locus of the <i>C. sporogenes</i> genome. This module comprises a strong clostridial terminator sequence T<i>fad</i> to prevent polar transcription from <i>pyrD</i> [https://parts.igem.org/Part:BBa_K2992013] and the <i>botR gene of<i>C. botulinum</i> [https://parts.igem.org/Part:BBa_K2992002], under the regulatory control of its native promoter P<i>botR</i> [https://parts.igem.org/wiki/Part:BBa_K2992012] and its associated 5’-UTR which contains the RBS [https://parts.igem.org/Part:BBa_K2992014]. An additional strong clostridial terminator was included to prevent polar effects for the transcription of<i> pyrE</i> and any downstream genes on the chromosome of <i>C. sporogenes</i>. In our project we use the transcriptional regulator BotR to control the regulation of our volatile reporter operons (hyperlink to comp parts) and our fluorescent reporter FAST [https://parts.igem.org/Part:BBa_K2992000] through interaction with its own promoter sequence P<i>botrR</i> [https://parts.igem.org/Part:BBa_K2992012] and P<i>ntnH</i> [https://parts.igem.org/Part:BBa_K2992001] whose genes are cognate members of the BotR regulon. Doing so allows us to use our surrogate host strain <i>C. sporogenes</i> as a model system for predicting botulinum neurotoxin production following food manufacture, through the detection of our chosen reporters. <br><br>
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===Characterisation===
 
===Characterisation===
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Data incoming. <br> <br>
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Data incoming  
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<partinfo>BBa_K2992025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992025 SequenceAndFeatures</partinfo>
  
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===References===
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===References===  
 
Cañadas et al., 2019 RiboCas – update
 
Cañadas et al., 2019 RiboCas – update
 
Dupuy  and Matamouros, 2006 update  
 
Dupuy  and Matamouros, 2006 update  
 
Minton et al., 2016 Road map – update
 
Minton et al., 2016 Road map – update
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Raffestin et al 2009 - update
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Raffestin et al 2009 update
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Dupuy  and Matamouros, 2006 update
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:24, 18 September 2019


No part name specified with partinfo tag.

Usage and Biology

This parts entry represents an integration module for the expression of botR from the pyrE locus of the C. sporogenes genome. This module comprises a strong clostridial terminator sequence Tfad to prevent polar transcription from pyrD [1] and the botR gene of<i>C. botulinum [2], under the regulatory control of its native promoter PbotR [3] and its associated 5’-UTR which contains the RBS [4]. An additional strong clostridial terminator was included to prevent polar effects for the transcription of pyrE and any downstream genes on the chromosome of C. sporogenes. In our project we use the transcriptional regulator BotR to control the regulation of our volatile reporter operons (hyperlink to comp parts) and our fluorescent reporter FAST [5] through interaction with its own promoter sequence PbotrR [6] and PntnH [7] whose genes are cognate members of the BotR regulon. Doing so allows us to use our surrogate host strain C. sporogenes as a model system for predicting botulinum neurotoxin production following food manufacture, through the detection of our chosen reporters.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cañadas et al., 2019 RiboCas – update Dupuy and Matamouros, 2006 update Minton et al., 2016 Road map – update Raffestin et al 2009 update