Difference between revisions of "Part:BBa K3168002:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
−
deep-sea shrimp derived luciferase
+
Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016).
  
 
===References===
 
===References===

Latest revision as of 12:52, 17 September 2019


LargeBitNanoLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A flexible (GGS)5 linker is located in front of the large bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.

Source

Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016).

References

Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.

Ohmuro-Matsuyama, Y., & Ueda, H. (2019). Protein-Protein Interaction Assays Using Split-NanoLuc. In Bioluminescence. IntechOpen.