Difference between revisions of "Part:BBa K3168001:Design"
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===Source=== | ===Source=== | ||
| − | Mutated variant of S. pyogenes derived Cas9, which was synthesised in gBlocks by IDT. | + | Mutated variant of S. pyogenes derived Cas9, which was synthesised in gBlocks by IDT. The sequence was based on Addgene plasmid #119808. |
===References=== | ===References=== | ||
Revision as of 12:47, 17 September 2019
dCas9-CP1041
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2143
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 261
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed for bacterial expression.
Source
Mutated variant of S. pyogenes derived Cas9, which was synthesised in gBlocks by IDT. The sequence was based on Addgene plasmid #119808.
References
Oakes, B. L., Fellmann, C., Rishi, H., Taylor, K. L., Ren, S. M., Nadler, D. C., ... & Savage, D. F. (2019). CRISPR-Cas9 circular permutants as programmable scaffolds for genome modification. Cell, 176(1-2), 254-267.
Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. PloS one, 12(6), e0179410.
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.
Sternberg, S. H., LaFrance, B., Kaplan, M., & Doudna, J. A. (2015). Conformational control of DNA target cleavage by CRISPR–Cas9. Nature, 527(7576), 110.