Difference between revisions of "Part:BBa K2992023"

 
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Component of the divergent P<i>bgaR</i> -P<i>bgaL</i> promoter predicted to regulate <i>bgaL</i> in <i>C. perfringens</i>.   
 
Component of the divergent P<i>bgaR</i> -P<i>bgaL</i> promoter predicted to regulate <i>bgaL</i> in <i>C. perfringens</i>.   
  
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===Usage and Biology===
 
===Usage and Biology===
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The BgaR-BgaL system of <i>C. perfringens</i>  comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent P<i>gaR</i> -P<i>gaL</i>  promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the promoter region predicted to regulate <i>bgaL</i>. Our group has recently utlised the BgaRL regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus <i>Clostridium</i> (Cañadas et al., 2019). In our project, we use the P<i>gaR</i> -P<i>gaL</i> regulatory system comprised of the divergent promoter and associated 5’-UTRs in conjunction with their cognate transcriptional regulator <i>bgaR</i>  (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture. <br><br>
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===Characterisation===
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Data incoming
  
 
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<partinfo>BBa_K2992023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992023 SequenceAndFeatures</partinfo>
  
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===References===
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Cañadas et al., 2019 RiboCas - update
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Hartman and Melville 2011.
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Zuker, 2003
  
 
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Revision as of 12:42, 17 September 2019


PbgaL component of PbgaR -PbgaL from C. perfringens.

Component of the divergent PbgaR -PbgaL promoter predicted to regulate bgaL in C. perfringens.

Usage and Biology

The BgaR-BgaL system of C. perfringens comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent PgaR -PgaL promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the promoter region predicted to regulate bgaL. Our group has recently utlised the BgaRL regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium (Cañadas et al., 2019). In our project, we use the PgaR -PgaL regulatory system comprised of the divergent promoter and associated 5’-UTRs in conjunction with their cognate transcriptional regulator bgaR (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cañadas et al., 2019 RiboCas - update Hartman and Melville 2011. Zuker, 2003