Difference between revisions of "Part:BBa K3168006:Design"
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===Design Notes=== | ===Design Notes=== | ||
A flexible (SGG)2 linker is located in front of NanoLuc to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification. | A flexible (SGG)2 linker is located in front of NanoLuc to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Arts, R., Aper, S. J., & Merkx, M. (2017). Engineering BRET-sensor proteins. In Methods in enzymology (Vol. 589, pp. 87-114). Academic Press. | ||
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| + | England, C. G., Ehlerding, E. B., & Cai, W. (2016). NanoLuc: a small luciferase is brightening up the field of bioluminescence. Bioconjugate chemistry, 27(5), 1175-1187. | ||
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| + | Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., Wood, M. G., ... & Robers, M. B. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS chemical biology, 7(11), 1848-1857. | ||
Revision as of 14:34, 16 September 2019
Cysteine-free-NanoLuc
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A flexible (SGG)2 linker is located in front of NanoLuc to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
Source
NanoLuc is a deep-sea shrimp derived luciferase (England, 2016)
References
Arts, R., Aper, S. J., & Merkx, M. (2017). Engineering BRET-sensor proteins. In Methods in enzymology (Vol. 589, pp. 87-114). Academic Press.
England, C. G., Ehlerding, E. B., & Cai, W. (2016). NanoLuc: a small luciferase is brightening up the field of bioluminescence. Bioconjugate chemistry, 27(5), 1175-1187.
Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., Wood, M. G., ... & Robers, M. B. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS chemical biology, 7(11), 1848-1857.