Difference between revisions of "Part:BBa K2992015"

Revision as of 12:49, 16 September 2019

5-UTR containing RBS for ntnH gene from C. botulinum

5’-UTR containing RBS for ntnH gene from C. botulinum

Usage and Biology

The native 5’-UTR containing the RBS is found naturally upstream of ntnH (BBa_K2992001) in C. botulinum. ntnH encodes the non-toxic non-haemagglutinin component of the botulinum neurotoxin complexes. Its expression is directly regulated by BotR (BBa_K2992002). In our project we use the regulatory region of ntnH to drive expression of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Raffestin et al., 2005 (update)

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 ===Usage and Biology===
-The native 5’-UTR containing the RBS is found naturally upstream of <i>ntnH</i> in <i>C. botulinum</i>. ntnH encodes the non-toxic non-haemagglutinin component of the botulinum neurotoxin complexes. Its expression is directly regulated by BotR.  In our project we use the regulatory region of <i>ntnH</i> to drive expression of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
+The native 5’-UTR containing the RBS is found naturally upstream of <i>ntnH</i> ([https://parts.igem.org/Part:BBa_K2992001 BBa_K2992001]) in <i>C. botulinum</i>. ntnH encodes the non-toxic non-haemagglutinin component of the botulinum neurotoxin complexes. Its expression is directly regulated by BotR ([https://parts.igem.org/Part:BBa_K2992002 BBa_K2992002]).  In our project we use the regulatory region of <i>ntnH</i> to drive expression of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
 ===Characterisation===