Difference between revisions of "Part:BBa K2992013"
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===Usage=== | ===Usage=== | ||
| − | T<i>fad</i> is a terminator sequence derived from the <i>fad</i> gene of <i>C. tetani</i>, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the <i>pyrE</i> operon of <i>C. sporogenes</i> following chromosomal knock-in of <i>botR</i> ( | + | T<i>fad</i> is a terminator sequence derived from the <i>fad</i> gene of <i>C. tetani</i>, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the <i>pyrE</i> operon of <i>C. sporogenes</i> following chromosomal knock-in of <i>botR</i> ([https://parts.igem.org/Part:BBa_K2992002 BBa_K2992002]) at this locus. The secondary structure for T<i>fad</i> was predicted using the Mfold web tool (Zuker, 2003). |
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Revision as of 12:25, 16 September 2019
Usage
Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrE operon of C. sporogenes following chromosomal knock-in of botR (BBa_K2992002) at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).
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Tfad from C. tetani
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Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]